TB-500 {Thymosin Beta-4} 5MG

Thymosin Beta 4

Thymosin Beta 4 (TB500) is a highly conserved, multifunctional peptide with broad regenerative and reparative properties. It is the most abundant β-thymosin in human cells, acting as a major G-actin–sequestering molecule and modulator of cytoskeletal dynamics.^[1][2] TB4 is released by platelets and other cell types at sites of injury, where it helps initiate and orchestrate the repair cascade.^[3]

Regenerative Properties & Wound Healing

• Accelerates dermal and epithelial wound healing in preclinical models and clinical settings (e.g., chronic ulcers, severe dermal injuries).^[4][5][3]
• Promotes keratinocyte/epithelial migration, mobilizes and supports stem/progenitor cells, and increases angiogenesis—key processes for rapid tissue repair.^{[4][5][2][6][7][3][8]}
• Inhibits apoptosis and dampens inflammation at wound sites, improving healing outcomes and tissue quality.^[4][5][6][3]

Inflammation Reduction & Tissue Repair

• Downregulates inflammatory chemokines/cytokines (including NF-κB pathways) and blocks steroid-induced thymocyte apoptosis, reducing local/systemic inflammation.^[9][6][3]
• The Ac-SDKP domain contributes anti-inflammatory and anti-fibrotic activity; utility noted in conditions with excessive inflammation or impaired repair (e.g., UC, liver/renal fibrosis, corneal injury).^{[6][10][1][11][3]}

Cell Migration & Angiogenesis

• Actin-binding motif (LKKTETQ) drives endothelial migration, adhesion, tubule formation, and angiogenesis.^[7]
• Stimulates vaso/angiogenesis and arteriogenesis in development and adult repair, enhancing perfusion and survival of injured tissues; upregulates matrix and remodeling programs.^{[9][12][7][3][2][6]}

Injury Recovery & Scar Modulation

• Improves recovery after myocardial infarction, I/R injury, stroke, and trauma by activating epicardial progenitors, supporting cardiomyocyte survival, and moderating inflammatory injury.^{[11][9][12][3]}
• Reduces myofibroblast burden and fibrosis → less scar formation; supports restoration of normal tissue architecture in dermal/corneal models.^[3][4][5][8]

Cardiovascular Support

• Essential for myocardial vascularization; improves perfusion and function in acute/chronic ischemic heart disease via survival kinases, endothelial/myocyte protection, and epicardial progenitor differentiation.^[9][12][3]

Summary: TB4 (TB500) is a cytoskeleton-modulating repair peptide that accelerates wound closure, reduces inflammation and fibrosis, promotes angiogenesis and progenitor activity, and supports recovery after ischemic and traumatic injury across multiple tissues.^[1–12]

References

1. Ying Y, Lin C, Tao N, et al. Curr Protein Pept Sci. 2023;24(1):78–88.
2. Crockford D, Turjman N, Allan C, Angel J. Ann N Y Acad Sci. 2010;1194:179–89.
3. Goldstein AL, Hannappel E, Sosne G, Kleinman HK. Expert Opin Biol Ther. 2012;12(1):37–51.
4. Treadwell T, Kleinman HK, Crockford D, et al. Ann N Y Acad Sci. 2012;1270:37–44.
5. Yang WS, Kang S, Sung J, Kleinman HK. Eur J Dermatol. 2019;29(5):459–467.
6. Sosne G, Qiu P, Goldstein AL, Wheater M. FASEB J. 2010;24(7):2144–51.
7. Philp D, Huff T, Gho YS, Hannappel E, Kleinman HK. FASEB J. 2003;17(14):2103–5.
8. Philp D, Kleinman HK. Ann N Y Acad Sci. 2010;1194:81–6.
9. Hinkel R, Trenkwalder T, Kupatt C. Ann N Y Acad Sci. 2012;1269:102–9.
10. Xing Y, Ye Y, Zuo H, Li Y. Front Endocrinol. 2021;12:767785.
11. Goldstein AL, Kleinman HK. Expert Opin Biol Ther. 2015;15(Suppl 1):S139–45.
12. Bjørklund G, Dadar M, Aaseth J, Chirumbolo S. Curr Med Chem. 2020;27(37):6294–6305.

All COA’s avail upon request by email info@truformlabs.com.

Storage & Handling (Research Use)

• Lyophilized (dry) peptide: Store sealed at −20 °C (long-term). Short-term storage up to 2–3 weeks at 2–8 °C is acceptable.
• After reconstitution: Store at 2–8 °C and use within 7 days, or aliquot to sterile vials and freeze at −20 °C for up to 3 months.
• Light & moisture: Protect from light; keep container tightly closed to prevent moisture uptake.
• Freeze–thaw: Avoid repeated freeze–thaw (prepare small aliquots).
• Solvent compatibility: Use a sterile solvent compatible with your protocol; filter-sterilize if required.
• Labeling: Clearly label aliquots with concentration, solvent, and preparation date.

Storage guidance is general for research peptides and may be adjusted per your lab SOP.